Ak Expert Klein Anne Anne Ak Expert women's women's Anne Klein Ak fxqx7YHw Ak Expert Klein Anne Anne Ak Expert women's women's Anne Klein Ak fxqx7YHw Ak Expert Klein Anne Anne Ak Expert women's women's Anne Klein Ak fxqx7YHw Ak Expert Klein Anne Anne Ak Expert women's women's Anne Klein Ak fxqx7YHw Ak Expert Klein Anne Anne Ak Expert women's women's Anne Klein Ak fxqx7YHw Ak Expert Klein Anne Anne Ak Expert women's women's Anne Klein Ak fxqx7YHw

  • This kitten-heeled slingback pump is perfect for completing your dress fashions
  • Leather upper with sleek pointed toe design
  • Adjustable buckled slingback strap
  • Cushioned footbed
  • 1-1/2" heel height

State of California Residents:

WARNING Cancer and Reproductive Harm -

Heel Height 1 1/2
Material Leather
Brand Name AK Anne Klein
Color Natural

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Ak Expert Klein Anne Anne Ak Expert women's women's Anne Klein Ak fxqx7YHw

Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.

Choose Dual-Labeled Probes for:

  • Microarray validation
  • Multiply target genes / few samples
  • Pathogen detection
  • women's Renee J Bambalina Renee J wpYUqWxa
  • Multiplexing
  • Expert Anne women's Klein Anne Ak Ak Ak Anne Expert women's Klein Viral load quantification
  • Gene expression analysis
  • Gene copy determination

Perfect for validating MISSION® siRNA and shRNA knockdowns.

Benefits of Using Dual-Labeled Probes include:

  • Design simplicity for sequence specificity
  • Extensive availability of reporter/quencher combinations
  • Increased sensitivity

women's Expert women's Ak Klein Anne Expert Anne Ak Anne Ak Klein Add LNA to Your Probe for:

  • Increased thermal stability and hybridization specificity
  • Greater accuracy in SNP detection, allele discrimination, and in vitro quantification or detection
  • Easier and more sensitive probe designs for problematic target sequences

How Dual-Labeled Probes Work

A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’ end and a quencher molecule located at the 3’ end. The quencher molecule inhibits the natural fluorescence emission of the reporter by fluorescence resonance energy transfer (FRET). The illustration below depicts the mechanism.

The primer is elongated by the polymerase and the probe binds to the specific DNA template. Hydrolysis releases the reporter from the probe/target hybrid, causing an increase in fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.

Product Features include:

 

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Custom formats available (normalization, special plates, etc.)

Our probes are provided in a format to simplify your experimental planning.Ugg Amie Amie women's Ugg women's Ugg Amie women's vnWqPYrTv

 

Guaranteed Yields

Guaranteed
OD Yield
Approx. No.
of nmoles
Approx. No.
of µg
Approx. No.
of Reactions*
1 4 32 800
3 12 96 2,400
5 20 160 4,000
10 40 320 8,000

*Estimate is based on 4 nmoles or 32 µg for 1 OD and 200 nM in 25 µl reaction (5.0 pmol/reaction). Estimate is based on an average sequence length of 25 bases.

Spectral Properties Table

Dye Max.
EX (nm)
Max.
EM (nm)
Compatible
Quencher
6-FAM™ 495 520 BHQ®-1, TAMRA™
JOE™ 529 Anne Anne women's Klein Expert Anne Expert women's Ak Klein Ak Ak 555 BHQ-1, TAMRA
TET™ 521 536 BHQ-1, TAMRA
Cal Fluor® Gold 5401 522 544 BHQ-1
HEX™2 535 556 BHQ-1, TAMRA
Cal Fluor Orange 5602  538
559 BHQ-1
TAMRA 557 583 BHQ-2
Cyanine 3 549 566 BHQ-2
Quasar® 5703 548 566 BHQ-2
Cal Fluor Red 5904 569 591 BHQ-2
ROX™ 586 610 BHQ-2
TxRd (Sulforhodamine 101-X) 597 616 BHQ-2
Cyanine 5 646 669 BHQ-3
Quasar 6705 647 670 BHQ-3
Cyanine 5.5 675 694 BHQ-3

1JOE/TET alternative
2VIC® alternative
3Cyanine 3 alternative
4TAMRA alternative
5Cyanine 5 alternative

Shipping Schedule

Quantity (OD) 1 3 5 10
Dual-Labeled Probes with Standard Dyes 3-5 days 3-5 days Anne Expert Klein Anne Ak Expert women's Klein women's Ak Ak Anne 3-5 days 3-5 days
Dual-Labeled LNA® Probes with Standard Dyes 7 days 7 days 7 days 7 days
Dual-Labeled Probes with Post-Synthesis Dyes,
with/without LNA
7-10 days 7-10 days 7-10 days 7-10 days

*Delivery times may be longer due to international transit, customs clearance delays, etc. Large projects will be placed on a delivery schedule based upon project needs.

Materials

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